Chagas disease is endemic in Latin America due to the presence of blood-sucking triatomine bugs (Hemiptera, Reduviidae) that transmit the parasite to vertebrate hosts 1. The World Health Organization (WHO) estimates 6–7 million people to be infected and approximately 75 million people are at risk of infection with this parasite worldwide. Trypanosoma cruzi is an intracellular protozoan parasite, the etiologic agent of American trypanosomiasis or Chagas disease. These results contribute to a better understanding of the EV-cell interaction and support the role of EV as virulence factors. Finally, a protective role of EV over apoptosis is suggested, as relative values of cells in early and late apoptosis were significantly lower in EV-treated cells, which also showed increased CSNK1G1 expression. Moreover, the expression of Rho-GTPases ( RhoA, Rac1 and Cdc42) after the interaction was analyzed, revealing a downregulation of the analyzed genes after 4 h of interaction. In this regard, the overexpression of genes related to ubiquitin-related processes ( Ube2C, SUMO1 and SUMO2) is highlighted. Results after EV-cell incubation revealed 322 differentially expressed genes (168 were upregulated and 154 were downregulated). In this work, we present a transcriptomic analysis of cells stimulated with EV of the trypomastigote stage of T. The pathogenesis of the disease is multifactorial and involves the virulence of the strains, immunological factors and extracellular vesicles (EV) shed by the parasite which participate in cell–cell communication and evasion of the immune response. The disease has an acute and a chronic phase in which approximately 30% of the chronic patients suffer from heart disease and/or gastrointestinal symptoms. Chagas disease is caused by the protozoan parasite Trypanosoma cruzi.
0 Comments
Leave a Reply. |